My wife took courses to renew her medical technologist license last year

It depends.

Does she work as an employee or does she have her own Self-employed business?

Assuming this is part of a Self-employed business that you are running, you would record the expenses in the federal interview section of the program as follows:

1. Select Income & Expenses 
2. Select Self-employment income and expenses 
3. Proceed through the screens to enter your information regarding your business. 
4. You will be able to add expenses in this section as well as your income

If however, this is an unreimbursed expense incurred as an employee, you will not be able to deduct it on your federal return. However, if you are allowed to itemize your deductions on your state return, you may be able to deduct it.

You will enter these deductions in the Federal section of the return. 

1. Go to Deductions & Credits 
2. Under  All breaks, select Employment Expenses
3. Select Job Expenses for W-2 Income 
4. Proceed to enter the applicable expenses. 

Enter all of your information in the federal section to ensure it gets pulled into your state return.